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            RTD6110 彩虹預染超低分子量蛋白Marker(1.2-45KD)
             產品貨號: RTD6110
             產品名稱: RTD6110 彩虹預染超低分子量蛋白Marker(1.2-45KD)
             產品價格/規格: 10次 500元
             產品說明書: 點擊查看
             更新時間: 2022-04-11
            詳細信息   訪問次數:

            • 產品介紹
            • 發表文章
            • 相關產品
            • 質檢證書
            • 彩虹預染超低分子量蛋白質Marker1.2-45kD

              Rainbow Prestained Ultra Low Molecular Weight Protein Marker

               

              產品編號及規格:

              RTD6110   10T50μl)   500元

              儲存條件:

                -20保存,開封后有效期12個月

              產品簡介:

                  彩虹預染超低分子量蛋白質Marker可以直接觀察蛋白電泳及清晰判斷Western Blotting的轉膜效果。本產品包含3種多肽和3種低分子量蛋白質組成,分子量范圍為1.2kD-45kD,分子量大小為  1.2kD,4.6kD,10kD,16kD,27kD,45kD (注:蛋白大小已在18%Tricine-SDS-PAGE中用非預染蛋白Marker標定過。




              ● 使用說明:

              第一次收到該產品,常溫融化后,徹底混勻,離心快甩將溶液完全收集到管底。

              . 制膠:

              I 配制分離膠

              1.按照表一將不同體積的雙蒸水、40% PAA19:1)、4×凝膠緩沖液(貨號:GB010)和乙二醇加入到小燒杯中混合。

              2.加入10%APSTEMED,立即混勻5-10秒,以使溶液充分混勻。

              3.在凝膠模具中迅速灌入適量分離膠溶液,然后在分離膠溶液上輕輕覆蓋一層1 cm的無水乙醇,使凝膠表面保持平整。

              4.靜置15-30分鐘,待分離膠和乙醇層之間出現一個清晰的界面表示凝膠已聚合。

              表一  (一塊1 mm mini膠用量)

               

              分離膠

              濃縮膠

               

              18T, 5%C /5 ml

              5T, 3.3%C/2 ml

              40% PAA19:1

              2.25 ml

              /

              40% PAA (29:1)

              /

              0.25 ml

              4×凝膠緩沖液

              1.25 ml

              0.5 ml

              乙二醇(電泳級)

              1.5 ml

              /

              ddH2O

              /

              1.25ml

              10APS

              50 μl

              20 μl

              TEMED

              5 μl

              2 μl


              II 配制濃縮膠

              去除覆蓋在分離膠上的乙醇層,用濾紙將殘留乙醇吸去。

              1.按照表一將不同體積的雙蒸水、40%PAA(29:1)和4×凝膠緩沖液加入到小燒杯中混合。

              2.加入10%過硫酸銨和TEMED,立即混勻5-10秒,以使溶液充分混勻。

              3.將梳子插入凝膠內,避免產生氣泡。

              4.靜置30-60分鐘裝待凝膠聚合

              . 電泳:

              1. Marker樣品,充分混勻后備用。不要加熱處理。

              2. 電泳前,將10×陽極緩沖液(Cat No:AB080)和10×陰極緩沖液(Cat No:CB010)用蒸餾水稀釋成1×緩沖液備用。將電泳槽的外槽加入1×陽極緩沖液,內槽加入1×陰極緩沖液,輕柔拔出梳子,將Marker或蛋白樣品(已經過2×Tricine上樣緩沖液[Cat No:TP050]處理)加入點樣孔,參考下表進行電泳,至每條帶都清晰可見時停止電泳。整個電泳過程大約需要2-3個小時。

              表二 多肽電泳條件

              恒電壓

              150V

              起始電流

              60-75mA/板膠

              結束電流

              15-25mA/板膠

              電泳時間

              2.5-3小時

              . 染色

              根據常規實驗步驟,用配方6和7(表三)進行染色和觀察。如果使用配方6進行染色時效果不好或考慮其毒性,請選擇本公司的FastBlue蛋白染色液(貨號:RTD6202),該產品除具有染色快,無毒,靈敏性高等特點外,還能對小肽在染色中起到固定作用,不至于小肽在染色和脫色中從凝膠中脫離,是常規染色液的理想替代品。



               相關產品:

              多肽電泳配套試劑

              名稱

              貨號

              規格

              超低分子量蛋白電泳試劑盒

              RTD6120

              10

              超低分子量蛋白Marker I3.3-20.1 kD

              RTD6101

              10次(100 μl

              超低分子量蛋白Marker III3.4-100 kD

              RTD6125

              10次(50 μl

              彩虹預染超低分子量蛋白Marker1.2-45 kD

              RTD6110

              10 50 μl

              彩虹預染超低分子量蛋白Marker II3-40 kD

              RTD6145

              10 50 μl

              50次(250 μl

              40% PAA19:1

              AC1914-01

              100ml

              40% PAA29:1

              AC2914-01

              100ml

              多肽電泳凝膠緩沖液

              GB010

              100ml

              10×Tris-Tricine-SDS電泳緩沖液(干粉型)

              CB010P

              500ml

              2×Tricine 上樣緩沖液

              TP050

              10×1ml

              FastBlue蛋白染色液

              RTD6202

              500ml

              乙二醇(電泳級)

              EA0582-02

              100 ml

              10×陽極緩沖液 (多肽電泳用

              AB080

              250 ml

              10×陰極緩沖液(多肽電泳用)

              CB010

              100 ml

               





            • 使用蛋白Marker產品發表部分文章列表:

              1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieaeisolated from traditional Chinese fermented cucumber.

              Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang

              Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

              Journal: Food Control 50 (2015) 896e900

              Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

              Paper linkhttp://dx.doi.org/10.1016/j.foodcont.2014.10.040

               

              2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by   isolated from fermented sausage Lactobacillus plantarum.

              Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie

              Zhang, Changqing Yu, Xue Han

              Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

              Journal: Food Control  Volume 81, November 2017, Pages 211-217

              Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

              Paper linkhttps://doi.org/10.1016/j.foodcont.2017.05.030

               

              3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.

              Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu

              Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

              Journal: Frontiers in Plant Science.  Volume 81, November 2017, Pages 211-217

              InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

              Paper linkhttps://doi.org/10.1093/jxb/ery351

               

              4. [2021 IF=2.99] Purifcation, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan Tsai.

              Author: Xinran Lv,  Yang Lin ,Yu Jie, Mengtong Sun, Bolin Zhang,Fengling Bai,Hongfei Zhao,Jianrong Li

              Marker: RTD6127,超低分子量蛋白Marker IV(3.3-45 kD)

              Journal: European Food Research and Technology. Vol 244, pages 323–331 (2018)

              InstitutionCollege of Biological Science and Biotechnology, Beijing Forestry University

              Paper linkhttps://link.springer.com/article/10.1007/s00217-017-2958-3

               

              5. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.

              Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu

              Marker: RTD6110,彩虹超低分子量蛋白Marker (1.2-45 kD)

              Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019,613-626

              InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

              Paper linkhttps://doi.org/10.1093/jxb/ery351

               

              6. [2021 IF=11.277] An  Alternative  Splicing  Variant  of  PtRD26  Delays  Leaf  Senescence by Regulating Multiple NAC Transcription Factors in Populus.

              Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li

              Marker: RTD6110,彩虹預染超低分子量蛋白(1.2-45 kD)

              Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614

              InstitutionBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University

              Paper linkhttps://doi.org/10.1093/plcell/koab046

               

              7. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

              Author: Feng Qin, Shujuan Wang, Mingxia Gao  and  Xiangmin Zhang

              Marker: RTD6110,彩虹預染超低分子量蛋白(1.2-45 kD)

              Journal: Analytical Methods. 2021,13, 2804-2811

              InstitutionDepartment of Chemistry, Fudan University

              Paper linkhttps://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b

               

              8. [2021 IF=3.74] Cloning, expression, and function of ferritins in the tick Haemaphysalis flava.

              Author: Yu Zhao, Lei Liu, Jin-Bao Liu, Cong-Ying Wu, De-Yong Duan, Tian-Yin Cheng

              Marker: RTD6142 高分子量非變性電泳蛋白質Marker II(45-669 kD)

              Journal: Ticks and Tick-borne Diseases. Volume 13, Issue 2, March 2022, 101892

              InstitutionCollege of Veterinary Medicine, Hunan Agricultural University,

              Paper linkhttps://linkinghub.elsevier.com/retrieve/pii/S1877959X21002454

               

              9. [2021 IF=0.886] Purification and Characterization of an Intracellular β-Glucosidase from the Protoplast Fusant of Aspergillus oryzae and Aspergillus niger

              Author: F.M. Zhu, B. Du, H.S. Gao, C.J. Liu, and J. Lia

              Marker: RTD6109 高分子量蛋白質Marker(43-200 kD)

              Journal: Applied Biochemistry and Microbiology, 2010, Vol. 46, No. 6, pp. 626–632

              InstitutionCollege of Food Science and Technology, Hebei Normal University of Science and Technology

              Paper linkhttps://link.springer.com/article/10.1134/S0003683810060116

               

              10. [2021 IF=4.374] Effect of Fuzhuan brick-tea addition on the quality and antioxidant activity of skimmed set-type yoghurt.

              Author: Dong Liu

              Marker: RTD6103 低分子量蛋白質Marker II(14.4-116 kD)

              Journal: International Journal of Dairy Technology, Vol 71 March 2018 Pages 22-33

              InstitutionDepartment of Food Processing Technology, Anqing Vocational & Technical College

              Paper linkhttps://doi.org/10.1111/1471-0307.12395

               

              11. [2021 IF=3.7] Smart delivery of poly-peptide composite for effective cancer therapy

              Author: Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu

              Marker: Protein Marker

              Journal: Biomedical Materials, 2022 Jan 24;17(2)

              Institution:School of Nano-Tech and Nano-Bionics, University of Science and Technology of China

              Paper linkhttps://pubmed.ncbi.nlm.nih.gov/34996052/

               



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