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            RTU4032 GUS染色試劑盒
             產品貨號: RTU4032
             產品名稱: RTU4032 GUS染色試劑盒
             產品價格/規格: 50ml/500元
             產品說明書: 點擊查看
             更新時間: 2022-04-06
            詳細信息   訪問次數:

            • 產品介紹
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            • 相關產品
            • 質檢證書
            • GUS染色試劑盒(貨號:RTU4032

              ●     產品組成:

              組分貨號

              名稱

              規格

              貯存

              運輸

              RTU4032-01

              X-gluc

              粉末

              -20

              RT

              RTU4032-02

              X-gluc溶解液

              1 ml

              -20℃

              RT

              RTU4032-03

              GUS染色緩沖液

              50 ml

              4

              RT

               

              說明書

              一份

               

               

              產品簡介:

              gus(β-glucuronidase,β-D- 葡萄糖苷酸酶)基因是目前常用的一種報告基因,其表達產物β-葡萄糖苷酸酶 (GUS)是一種水解酶,能催化許多β-葡萄糖苷酯類物質的水解,它可以將5-溴-4-氯-3-吲哚-β-葡萄糖苷酸酯(X-gluc.htm' target=_blank>x-gluc)分解為藍色的物質,其檢測方法簡單、快速、靈敏、穩定,且背景活性低。因為絕大多數植物細胞內不存在內源的GUS活性,因此gus基因廣泛用作轉基因植物的報告基因,尤其是在研究外源基因瞬時表達的轉化實驗中廣泛應用。

              該試劑盒包含GUS染色的全部試劑,使用方便,只需將配制好的X-gluc溶液和緩沖液按照比例混合即配成GUS染色液。該試劑盒可以配制50ml GUS染色液。

              貯存和效期:

              未配制的試劑盒按照溫度貯存,有效期2年。

              配制好的X-gluc溶液-20℃避光保存30天,長期-80℃保存;GUS染色緩沖液 4貯存;GUS染色工作液最好現用現配,短期貯存可以-20℃保存2-3

              使用說明:

              一、X-gluc溶液(50×)配制:

              吸取1ml X-gluc溶解液加入到X-gluc管中,徹底混勻,至粉末完全溶解,即配成X-gluc溶液(50×),該溶液-20℃避光保存。

              注:X-gluc溶液穩定性較差,-20℃避光保存30天左右溶液保持無色,長期保存建議按照需要分裝到棕色管里,-80℃避光保存。正常的X-gluc溶液顏色為無色,如果溶液變為紅色或棕色,表明溶液失效。


              .  GUS染色工作液配制:

               

              GUS染色工作液 配制量

               

              1 ml

              5 ml

              10 ml

              X-gluc溶液(50×)

              20 μl

              100 μl

              200 μl

              GUS染色緩沖液

              1 ml

              5 ml

              10 ml

              注:GUS染色工作液最好現用現配,短期貯存可以-20℃保存2-3天。

              . GUS染色步驟:

              1.取材:將葉片、花瓣、根莖等組織剪成小片,放于1.5ml離心管中。

              注:用于染色的植物材料的制備方法要因涉及的特定組織和器官的不同而異。例如,擬南芥的根、花和葉片以及煙草幼苗的根就可以不作任何預處理而直接染色。但是像煙草和馬鈴薯這些植物的莖和葉就必須在染色前切成薄片(1-3mm)。當操作大的組織和樣品時,可以選用真空滲入法來幫助底物滲入細胞。

              2.染色:加入適量配制好的GUS染色工作液至完全覆蓋材料;錫箔紙包好后搖床搖動10分鐘或更長時間至材料出現藍色。注意要做陰性和陽性對照實驗。 

              3.洗脫:將材料轉入無水乙醇或80%丙酮中脫色2-3次,至陰性對照材料為白色。GUS染色陽性的藍色斑點很穩定,在酒精中不褪色。

              4.觀察:一般染色后,GUS染色陽性的藍色斑點肉眼就能看到。但是有些材料可能藍色斑點很細微,要在顯微鏡下才能看到。

            • GUS染色試劑盒

              使用該產品發表部分文章列表:

              1. [IF=6.456] A PIF1/PIF3-HY5-BBX23 Transcription Factor Cascade  Affects Photomorphogenesis.

              Author: Xinyu Zhang, Fangfang Shang, Junling Huai, Gang Xu, Weijiang Tang, Yanjun Jing,Rongchen g Lin.

              Journal: Plant Physiology. 2017,Vol. 174, 2487–2500.

              Institution Institute of Botany,Chinese Academy of Sciences,Beijing,China

              Paper linkhttp://www.plantphysiol.org/content/174/4/2487

               

              2. [IF=6.17] PICKLE chromatin remodeling factorcontrols thermosensory hypocotyl growth of Arabidopsis. 

              Author: Zha P, Jing YJ, Xu, G, Lin RC

              Journal: Plant, Cell and Environment.2017,Vol40, 2426–36.

              Institution Institute of Botany,Chinese Academy of Sciences,Beijing,China

              Paper linkhttp://onlinelibrary.wiley.com/doi/10.1111/pce.13049/full

               

              3. [IF=4.3] The Wheat Bax Inhibitor-1 Protein Interacts withan Aquaporin TaPIP1 and Enhances Disease Resistance in Arabidopsis. 

              Author: Pan-Pan Lu,Tai-Fei Yu,Wei-Jun Zheng, Ming Chen, Yong-Bin Zhou,Jun Chen, You-ZhiMa, Ya-Jun Xi and Zhao-Shi Xu.

              Journal: Frontiers in Plant Science.2018,Vol.9

              InstitutionCollege of Agronomy, Northwest A&F University;

              Paper linkhttps://www.frontiersin.org/articles/10.3389/fpls.2018.00020/full

               

              4. [IF=3.33] Expression and functional analysis of two PsbS genes in bamboo (Phyllostachys edulis). 

              Author: Yongfeng Lou,Huayu Sun,Sining Wang,Hao Xu,Lichao Li,Hansheng Zhao,Zhimin Gao.

              Journal: Pysiol Plant2018

              Institution International Center for Bamboo andRattan, Beijing,China

              Paper linkhttp://onlinelibrary.wiley.com/doi/10.1111/ppl.12690/full

               

              5. [IF=3.154] MultiplePesticides Detoxification Function of Maize (Zea mays) GST34. 

              Author: Dongzhi Li, Li Xu, Sen Pang, Zhiqian Liu, Weisong Zhao, Chengju Wang.

              Journal: J. Agric. Food Chem2017 ,65 (9),pp 1847–1853

              InstitutionCollege of Science, China Agricultural University, Beijing,China

              Paper linkhttp://pubs.acs.org/doi/abs/10.1021%2Facs.jafc.7b00057

               

              6.[IF=2.87] The bambooaquaporin gene PeTIP4;1–1 confers drought and salinity tolerance in transgenicArabidopsis. 

              Author: Huayu Sun, Lichao Li, Yongfeng Lou, Hansheng Zhao, Yihong Yang, Sining Wang, Zhimin Gao.

              Journal: Plant Cell Rep  2017,Vol36, 4, 597–609

              Institution International Center for Bambooand Rattan, Beijing,China

              Paper linkhttps://link.springer.com/article/10.1007/s00299-017-2106-3

               

              7.[IF=2.236] Characterizationand Primary Functional Analysis of a Bamboo ZEP Gene from Phyllostachys edulis.

              Author: Yongfeng Lou, Huayu Sun, Lichao Li,Hansheng Zhao, Zhimin Gao.

              Journal: DNA and Cell Biology2017,Vol 36,9,1-12

              Institution International Center for Bambooand Rattan, Beijing,China

              Paper linkhttp://online.liebertpub.com/doi/abs/10.1089/dna.2017.3705

               

              8.[IF=3.687] Expression Pattern and Function Analysis of AtPPRT1, a NovelNegative Regulator in ABAand Drought Stress Responses in Arabidopsis

              Author: Linsen Pei, Lu Peng, Xia Wan, Jie Xiong, Zhibin Liu, Xufeng Li, Yi Yang andJianmei Wang.

              Journal: Int. J. Mol. Sci. 2019, 20, 394

              Institution College of Life Sciences, Sichuan University

              Paper linkhttps://www.mdpi.com/1422-0067/20/2/394

               

              9. [IF=3.93]  Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates responseto heat stress

              Author: Pan-Pan Lu, Wei-Jun Zheng, Chang-TaoWang, Wen-Yan Shi, Jin-Dong Fu, Ming Chen, Jun Chen,Yong-Bin Zhou, Ya-Jun Xi andZhao-Shi Xu.

              Journal: BMC Plant Biology 2018, 18,259

              InstitutionInstitute of Crop Science, Chinese Academy of Agricultural Sciences

              Paper link: https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1485-0

               

              10. [IF=5.415]  SpHMA1 is a Chloroplast Cadmium Exporter Protecting Photochemical Reactions in the Cd Hyperaccumulator Sedum plumbizincicola

              Author: Haixia Zhao  Liangsheng Wang  Fang‐Jie Zhao  Longhua Wu Anna Liu  Wenzhong Xu.

              Journal:  Plant, Cell and Environment  12 October 2018

              Institution Institute of Botany, Chinese Academy of Sciences,Beijing,China

              Paper link: https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13456

               

              11. [IF=8.827] SEUSS and PIF4 Coordinately Regulate Light and Temperature Signaling Pathways to Control Plant Growth.

              Author: Junling Huai, Xinyu Zhang, Jialong Li, Tingting Ma, Ping Zha, Yanjun Jing,Rongcheng Lin.

              Journal:  Molecular Plant 2018,11,928-942

              Institution Institute of Botany, Chinese Academy of Sciences,Beijing, China

              Paper link: https://doi.org/10.1016/j.molp.2018.04.005

               

              12. [IF=3.825]  AtARRE, an E3 ubiquitin ligase, negatively regulates ABA signaling in Arabidopsis thaliana.

              Author: Boya Wang,Chuzhe Li,Xiangge Kong, Ying Li, Zhibin Liu, Jianmei Wang, Xufeng Li, Yi Yang.

              Journal: Plant Cell Reports 37, 1269–1278 (2018)

              Institution College of Life Sciences, Sichuan University

              Paper link: https://doi.org/10.1007/s00299-018-2311-8

               

              13. [IF=6.902]  OsDER1 is an ER-associated protein degradation factor that responds to ER stress. Author: Dandan Qian, Guoqiang Chen, Lihong Tian, Le Qing Qu.

              Journal:  Plant Physiol 2018 178,402-412

              Institution Institute of Botany, Chinese Academy of Sciences

              Paper link: https://doi.org/10.1104/pp.18.00375

               

              14. [IF 3.93] Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress. Author: Pan-Pan Lu, Wei-Jun Zheng, Chang-Tao, Wang, Zhaoshi Xu.

              Journal:  BMC Plant Biology  18, 259 (2018)

              Institution Institute of Crop Science, Chinese Academy of Agricultural Sciences 

              Paper link: https://doi.org/10.1186/s12870-018-1485-0

               

              15. [IF 5.415] The chromatin-remodelling factor PICKLE interacts with CONSTANS to promote flowering in Arabidopsis.

              Author: Yanjun Jing, Qiang Guo, Ping Zha, Rongcheng Lin.

              Journal:  Plant, Cell and Environment 2019

              Institution Institute of Botany, Chinese Academy of Sciences, Beijing

              Paper link: https://doi.org/10.1111/pce.13557

               

              16. [IF 5.415] The  Arabidopsis  defensin  gene  AtPDF2.5  mediates  cadmium tolerance and accumulation.

              Author: Jin-Song Luo, Yong Yang, Tianyu Gu, Zhimin Wu, Zhenhua Zhang.

              Journal:  Plant, Cell and Environment  2019 May

              InstitutionHunan Agricultural University

              Paper link: https://doi.org/10.1111/pce.13592

               

              17. [IF 3.517] In Silico Genome-Wide Analysis of the Pear (Pyrus bretschneideri) KNOX Family and the Functional Characterization.

              Author: Xi Cheng, Yongping Cai.

              Journal: Frontiers in Genetics 2019,10 Article 632

              Institution:Anhui Agricultural University

              Paper link:  https://doi.org/10.3389/fgene.2019.00632

               

              18.[IF 3.929]  Chloroplast-Localized Protoporphyrinogen IX Oxidase1 Is Involved in the Mitotic Cell Cycle in Arabidopsis.

              Author: Jialong Li, Fan Zhang, Yuhong Li, Weicai Yang, Rongcheng Lin

              Journal: Plant and Cell Physiology 2019

              Institution Institute of Botany, Chinese Academy of Sciences, Beijing, China

              Paper link: https://doi.org/10.1093/pcp/pcz135

               

              19. [IF 3.259] Functional characterization of three MATE genes in relation to aluminum-induced citrate efflux from soybean root.

              Author: Ying Zhou, Zhengbiao Wang, Li Gong, Anle Chen, Ning Liu, Sha Li, Haoran Sun, Zhenming Yang, Jiangfeng You

              Journal: Plant Soil  443, 121–138 (2019)

              Institution:College of Plant Science, Jilin University

              Paper link: https://doi.org/10.1007/s11104-019-04192-w

               

              20. [IF 10.8] bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis.

              Author:RihuaLei, YangLi, YuerongCai, ChenyangLi, MengnaPu, ChengkaiLu, YujieYang, GangLiang.

              Journal: Molecular Plant Vol 13, Issue 4, 6 April 2020, 634-649

              Institution:Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences

              Paper link: https://doi.org/10.1016/j.molp.2020.01.006

               

              21. [IF 11.88] A mycorrhizae-like gene regulates stem cell and gametophore development in mosses.

              Author: ShuanghuaWang, Jinling Huang

              Journal: Nature Communications 2020,11,2030

              Institution:Kunming Institute of Botany, Chinese Academy of Sciences

              Paper link: https://doi.org/10.1038/s41467-020-15967-6

               

              22. [IF 4.1] Overexpression of a Defensin-Like Gene CAL2 Enhances Cadmium Accumulation in Plants .

              Author: Jin-Song Luo,Zhenhua Zhang. 

              Journal: Frontiers in Plant Science  2020

              InstitutionHunan Agricultural University

              Paper link: https://doi.org/10.3389/fpls.2020.00217

               

              23. [IF 1.644] AtPPRT1 negatively regulates salt stress response in Arabidopsis seedlings.

              Author: Yu Liu, Linsen Pei, Shuya Xiao, Lu Peng, Zhibin Liu, Xufeng Li, Jianmei Wang.

              Journal: Plant Signaling & Behavior  2020

              InstitutionSichuan University

              Paper link: https://doi.org/10.1080/15592324.2020.1732103

               

              24. [IF 3.824] Oryza Sativa Fer-Like Fe Deficiency Induced Transcription Factor (Osfit/Osbhlh156) Interacts With Osiro2 To Regulate Iron Homeostasis.

              Author: Gang Liang, Huimin Zhang, Yang Li, Mengna Pu, Yujie Yang, Chenyang Li, Chengkai Lu,  Peng Xu, Diqiu Yu.

              Journal:  J Integr Plant Biol  2020 ,Volume 62 ,Issue 5 ,668–689

              InstitutionXishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences

              Paper link: https://doi.org/10.1111/jipb.12933

               

              25. [IF暫無] RGG1, involved in the cytokinin regulatory pathway,controls grain size in rice.

              AuthorYajun Tao, Jie Yang 

              Journal: Reaearch Square 2020

              InstitutionJiangsu Academy of Agricultural Sciences

              Paper link: https://doi.org/10.21203/rs.3.rs-40598/v1


              26. [IF 4.0] Analysis of β-d-glucan biosynthetic genes in oat reveals glucan synthesis regulation by light.

              AuthorJing Zhang, Lin Yan, Minxuan Liu, Ganggang Guo and Bing Wu 

              Journal: Annals of Botany 2020  127: 371–380, 2021

              Institution:Institute of Crop Science, Chinese Academy of Agricultural Sciences

              Paper link: https://academic.oup.com/aob/article-abstract/127/3/371/5934920



              27. [IF 1.5] OsHIPP24 is a Copper Metallochaperone Which Affects Rice Growth.

              AuthorGuoqiang Chen,Shuo Xiong  

              Journal: Journal of Plant Biology Nov 30 2020 

              Institution:Institute of Botany, Chinese Academy of Agricultural Sciences

              Paper link: https://www.sciencedirect.com/science/article/abs/pii/S0006291X21005581



              28. [IF 8.154] Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV chalcone isomerase in citrus.

              AuthorChenning Zhao, Xiaojuan Liu , Qin Gong , Jinping Cao , Wanxia Shen , Xueren Yin , Donald Grierson , Bo Zhang   , Changjie Xu  , Xian Li , Kunsong Chen , Chongde Sun.

              Journal: Plant Biotechnology Journal  (2021) 19, pp. 671–688

              Institution:Zhejiang University

              Paper link: https://onlinelibrary.wiley.com/doi/10.1111/pbi.13494


              29. [IF 10.15] JMJ27-mediated histone H3K9 demethylation positively regulates drought-stress responses in Arabidopsis.

              AuthorQiongli Wang, Peng Liu, Hua Jing, Xiao Feng Zhou, Bo Zhao, Yuan Li, Jing Bo Jin

              Journal: New Phytologist  (2021)  01 July 2021

              Institution:IBCAS

              Paper link: https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.17593



              30. [IF 6.79] Plasma membrane-localized SlSWEET7a and SlSWEET14 regulate sugar transport and storage in tomato fruits.

              AuthorXinsheng Zhang, Chaoyang Feng, Manning Wang, Tianlai Li, Xin Liu, and Jing Jiang

              Journal: Horticulture Research  (2021) 8:186 

              Institution:College of Horticulture, Shenyang Agricultural University,

              Paper link: https://www.nature.com/articles/s41438-021-00624-w



              31. [IF 5.75] A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher K+/Na+ Ratio in Soybean.

              AuthorTianjie Sun,Jie Zhang, Jinfeng Cao and Dongmei Wang

              Journal: Frontiers in Plant Science  (2021) 12:638340

              Institution:College of Life Sciences, Hebei Agricultural University

              Paper link: https://www.frontiersin.org/articles/10.3389/fpls.2021.638340/full



              32. [IF 5.9]Expression Analyses of Soybean VOZ Transcription Factors and the Role of GmVOZ1G in Drought and Salt Stress Tolerance.

              AuthorBo Li 1,y, Jia-Cheng Zheng 2,y, Ting-Ting Wang 3,y, Dong-Hong Min 4, Wen-Liang Wei 3,Jun Chen 1, Yong-Bin Zhou 1, Ming Chen 1, Zhao-Shi Xu 1,* and You-Zhi Ma

              Journal: International Journal of Molecular Sciences  (2020) 21:2177

              Institution:Institute of Crop Science, Chinese Academy of Agricultural Sciences

              Paper link:https://pubmed.ncbi.nlm.nih.gov/32245276/



              33. [IF 7.228]Close arrangement of CARK3 and PMEIL affects ABA‐mediated pollen sterility in Arabidopsis thaliana.

              AuthorHsi-Hua Wang, Yao Qiu, Qin Yu, Qian Zhang, Xiaoyi Li, Jianmei Wang,Xufeng Li, Yang Zhang, Yi Yang

              Journal: Plant Cell and Environment 2020;43:2699–2711.

              Institution:Sichuan University

              Paper link:https://onlinelibrary.wiley.com/doi/10.1111/pce.13871


              34. [IF 5.92]PDF1.5 Enhances Adaptation to Low Nitrogen Levels and Cadmium Stress.

              AuthorZhimin Wu, Dong Liu, Ningyan Yue, Haixing Song, Jinsong Luo,and Zhenhua Zhang

              Journal: International Journal of Molecular Sciences  2021, 22, 10455..

              Institution:Hunan Agricultural University

              Paper link:https://www.mdpi.com/1422-0067/22/19/10455


              35. [IF 6.29]Repression of transcription factor AtWRKY47 confers tolerance to boron toxicity in Arabidopsis thaliana.

              AuthorYingna Feng, Rui Cui, Yupu Huang, Lei Shi, Sheliang Wang, Fangsen Xu

              Journal: Ecotoxicology and Environmental Safety 220(2021)112406

              Institution:Huazhong Agricultural University

              Paper link:https://www.sciencedirect.com/science/article/pii/S0147651321005182?via%3Dihub


              36. [IF 6.79]PpINH1, an invertase inhibitor, interacts with vacuolar invertase PpVIN2 in regulating the chilling tolerance of peach fruit.

              AuthorYingna Feng, Rui Cui, Yupu Huang, Lei Shi, Sheliang Wang, Fangsen Xu

              Journal:Horticulture Research ( 2020) 7:168

              Institution:Ningbo University

              Paper link:https://www.nature.com/articles/s41438-020-00389-8


              37. [IF 8.154]Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up-regulating the boric acid channel gene BnaA3.NIP5;1.

              AuthorYingna Feng, Rui Cui, Sheliang Wang, Mingliang He, Yingpeng Hua, Lei Shi, Xiangsheng Ye and Fangsen Xu

              Journal:Plant Biotechnology Journal  (2020) 18, pp. 1241–1254

              InstitutionHuazhong Agricultural University

              Paper link:https://onlinelibrary.wiley.com/doi/10.1111/pbi.13288


              38. [2021IF 5.92]An Efficient Marker Gene Excision Strategy Based on CRISPR/Cas9-Mediated Homology-Directed Repair in Rice.

              AuthorJiantao Tan, Yaxi Wang, Shuifu Chen, Zhansheng Lin, Yanchang Zhao, Yang Xue, Yuyu Luo,Yao-Guang Liu, Qinlong Zhu

              Journal:International Journal of Molecular Sciences,2022, 23(3), 1588

              InstitutionSouth China Agricultural University

              Paper link:https://www.mdpi.com/1422-0067/23/3/1588


              39. [2021IF 3.935]Identification and Functional Analysis of the CgNAC043 Gene Involved in Lignin Synthesis from Citrus grandis “San Hong”.

              AuthorXiaoting Li, Naiyu Wang, Wenqin She, Zhixiong Guo, Heli Pan, Yuan Yu , Jianwen Ye,Dongming Pan,Tengfei Pan

              Journal:Plants,2022, 11(3), 403

              Institution:Fujian Agriculture and Forestry University

              Paper link:https://www.mdpi.com/2223-7747/11/3/403/htm














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