Zeocin Selection Reagent

● 產品規格

Zeocin粉末型

● 產品說明

Zeocin™ Reagent is a member of the bleomycin antibiotic family. Resistance is conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cells expressing the protein, Zeocin™ is prevented from binding and cleaving the cellular DNA. Zeocin™ is effective on most aerobic cells and can be used for selection in mammalian and insect cell lines, yeast, and bacteria. The amount of Zeocin™ required for selection will vary between 50-2000 μg/ml (typically 300μg /ml) depending on the cell type.

Zeocin可選擇Sh ble基因表達的細胞。與現在使用的其他動物細胞標記物無交叉抗性。因此這種抗生素可用來分離對其他篩選劑（如：慶大霉素，潮霉素）有抗性的克隆。Zeocin是一種屬于爭光霉素家族的糖蛋白抗生素，在體內能作用于大多數細菌（包括E. coli）、真菌（如：酵母菌）、植物細胞、動物細胞。

● 液體Zeocin可用于細胞培養。

● 用于篩選轉化株的Zeocin濃度根據pH值和鹽濃度改變。pH越高，鹽濃度越低，Zeocin活性越大。

● 普通大腸桿菌菌株篩選可在25 μg/ml zeocin濃度的低鹽LB瓊脂培養基(yeast extract 5 g/L, Tryptone 10 g/L, NaCl 5 g/L, Agar 15 g/L,pH 7.5）中進行，pH調至7.5 。

● 動物細胞篩選：根據不同細胞系從100 到 750 μg/ml。

● 植物細胞篩選：根據不同的細胞種類和培養基從50 到 250 μg/ml 。

● 酵母篩選：取決于種屬和培養基，S. cerevisiae Sh ble轉化株在50 μg/ml 濃度的YPD培養基中篩選，pH調至7.0。

● 粉末型配制方法：

取1 ml Zeocin溶解液加到Zeocin干粉中，徹底溶解，用0.22 μm 一次性濾器抽濾除菌，即配成1 ml（100mg/ml）的Zeocin溶液，-20℃保存。

Empirical formula: C₅₅H₈₅O₂₁N₂₀S₂Cu·HCl

Molecular weight: 1525

CAS：11006-33-0

 ● Reference：

1. Drocourt, D., T. et al. 1990 Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower higher eukaryotes to phleomycin resistance. Nucl. Acids. Res. 18: 4009.

2. Gatignol,A. et al. 1988. Bleomycin resistance conferred a drug-binding protein. FEBS Letters. 230 : 171-175.

3. DUMAS, Ph. et al. 1994. The thr dimensional structure of a bleomycin resistance protein. EMBO J. 242 (5) 595-601.