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            TR4201S Timentin溶液 100mg/ml 無菌 即用型
             產品貨號: TR4201S
             產品名稱: TR4201S Timentin溶液 100mg/ml 無菌 即用型
             產品價格/規格: 10×1ml 400元
             產品說明書: 點擊查看
             更新時間: 2022-06-07
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            • Timentin溶液(100mg/ml)

              Timentin Solution100mg/ml

              產品包裝:

              產品編號

              產品名稱

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              說明書

              TR4201S

              Timentin溶液(100 mg/ml)

              10×1ml

              1

              CAS: 4697-14-7/61177-45-5(替卡西林鈉/克拉維酸鉀)

              Formula: C15H14N2Na2O6S2/C8H8KNO5

              Appearance: pale yellow clear Solution

              Typical Working Concentration: 50-200 mg/L

              產品簡介:

              Timentin is a suitable antibiotic for Agrobacterium counterselection and a mixture of two antibiotics: ticarcillin and clavulanic acid. Most wild Agrobacterium isolates have beta-lactamase activity which clavulanic acid exhibits activity towards. Many Agrobacterium isolates are highly susceptible to Timentin. Agrobacterium strains carrying the standard pBR beta-lactamase gene are also Timentin (clavulanic acid) susceptible. Timentin killing of Agrobacterium wild-type strains is three logs greater than with comparable doses of carbenicillin.

              In plant transformation experiments, 100 μg/ml Timentin is sufficient to counterselect Agrobacterium. To improve efficacy (of any antimicrobial), solid media are best slightly dried before use. At these concentrations, Timentin exhibits no phytotoxicity to Arabidopsis root cultures.

              保存條件:

                 -20保存。有效期12個月。

              References:

              1 Zimmerman TW (1995) Effect of Timentin for controlling Agrobacterium tumefaciens following cocultivation on select plant species. In Vitro Cell Dev Biol 31:70A

              2 Cheng Z M et al. Timentin as an alternative antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. Plant Cell Reports (1998) 17: 646–649



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