Formulation: 50 mg/ml in PBS (phosphate buffered saline), sterile filtered.
Formula: C20H37N3O13 CAS: 31282-04-9 MW: 527.5
Purity: 80% (HPLC)
Hygromycin B is an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus that kills
bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It
has been reported to interfere with translocation and to cause mistranslation. Hygromycin
B is used for the selection of pro- and eukaryotic cells, stably transfected
with the Hygromycin resistance gene, as well as for the maintenance of the
Hygromycin phenotype of resistant cells.
● Working concentration:
concentration for the selection of resistant cells is 50 –1000 μg/ml (see table
1). The optimal concentration is to be tested experimentally and may vary with
the cell type. A commonly used concentration for selection of mammalian cells
hygromycin B solution is stable at +2
to +8°Cfor at least
five years. Some customers have been reported that frozen solution have lost
complete activity. Since solutions are extremely stable refrigerated, there should be no need to freeze
A gene has been identified that confers resistance in E. coli against Hygromycin B. The
resistance gene codes fore kinase that inactivates Hygromycin B through
phosphorylation (Hygromycin B phosphotransferase). Cloning of the resistance
gene (designated HM+, HMR, hyg or hph) and fusion with
eukaryotic promoters has resulted in the construction of vectors that allow
selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cell
systems. A variety of vectors have been developed. Hygromycin B has been used
for selection of a wide variety of transfected cells (see table 1).
Table 1 Concentration of
Hygromycin B used for selection of cells after transfection with DNA constructs
encoding for Hygromycin resistance.