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            HR2080 Hygromycin B solution 潮霉素溶液
             產品貨號: HR2080
             產品名稱: HR2080 Hygromycin B solution 潮霉素溶液
             產品價格/規格: 20ml/800元
             產品說明書: 點擊查看
             更新時間: 2022-03-22
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            • Hygromycin B Solution

              潮霉素B即用型溶液

              Contents20ml

              Formulation: 50 mg/ml in PBS (phosphate buffered saline), sterile filtered.

              Formula:  C20H37N3O13  CAS: 31282-04-9 MW: 527.5

              Purity: 80% (HPLC)

              Storage4

              Application:

              Hygromycin B is an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It has been reported to interfere with translocation and to cause mistranslation. Hygromycin B is used for the selection of pro- and eukaryotic cells, stably transfected with the Hygromycin resistance gene, as well as for the maintenance of the Hygromycin phenotype of resistant cells.

              Working concentration:

              Recommended concentration for the selection of resistant cells is 50 –1000 μg/ml (see table 1). The optimal concentration is to be tested experimentally and may vary with the cell type. A commonly used concentration for selection of mammalian cells is 200μg/ml.

              Stability:

              The hygromycin B solution is stable at +2 to +8°Cfor at least five years. Some customers have been reported that frozen solution have lost complete activity[1]. Since solutions are extremely stable refrigerated, there should be no need to freeze them at-20.

              Resistance:

              A gene has been identified that confers resistance in E. coli against Hygromycin B. The resistance gene codes fore kinase that inactivates Hygromycin B through phosphorylation (Hygromycin B phosphotransferase). Cloning of the resistance gene (designated HM+, HMR, hyg or hph) and fusion with eukaryotic promoters has resulted in the construction of vectors that allow selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cell systems. A variety of vectors have been developed. Hygromycin B has been used for selection of a wide variety of transfected cells (see table 1).

              Reference

              1 Perl, A. et al., Nat. Biotechnol., 14, 624-628 (1996).

              Table 1 Concentration of Hygromycin B used for selection of cells after transfection with DNA constructs encoding for Hygromycin resistance.

              Species

              Cell Type

              Cell Line/strain

              HygromycinB [μg/ml]

              E. coli

               

              JM 83

              25–100

              200

              Streptomyces lividans

               

               

              50

              Saccharomyces cerevisiae

               

               

              200

              Aspergilus nidulans

               

              GR 1

              250

              GR 5

              250

              G 191

              250

              G B 20

              750

              W 1

              >1000

              FGSC 4

              >1000

              FGSC 237

              >1000

              Tobacco

               

               

              20–200

              Chicken

               

              DT 40

              1500

              27 C 2

              1500

              RP 9

              1500

              HD 3

              1500

              CU 39

              1500

              BM 2

              1500

              Mouse

              ES cells

              LTK

              200–400

              L 929

              200

              C 127

              300

              NIH 3T3

              50–500

              PA 317

              50

              ψ2

              150

              ΩE

              50

              120

              Rat

              Embryo fibroblasts

               

              200

              Human

               

              B 95–8

              50

              Raji

              100

              721

              100

              WIL 2 TK-/-

              200

              Daudi

              200

              GG 68

              400

              293

              200

              K 562

              200

              Mouse-human

              hybrid cell

              SCC 16-5

              200

               




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